Part:BBa_K2705006:Design

P_gltAB-LacI-P_grac-TetA

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1229
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

It was applied to sieve Bacillus amyloliquefaciens LL3 bacteria with high level glutamate under tetracycline circumstance, which can produce more products whose precursor is glutamate such as γ-PGA. Since LL3 can produce glutamate by itself, high-producing cells can survive based on the amount of precursor.

Source

P_gltAB was acqcuired from LL3 by PCR, LacI was from vector pTH01 by PCR, P_grac was from vector pTH01 by PCR, TetA was from vector pWH1520 by PCR.